Active spatial positioning of condensates on membranes by self-organizing proteins
Prof. Petra Schwille, MPI of Biochemistry
Project: The project is part of the 
Condensates at Membrane Scaffolds – Integrated Systems as Synthetic Cell Compartments (ComeInCells), a European Doctoral Network at the forefront of synthetic cell research. Applicants must not have lived in Germany for more than 12 months within the last three years (EU mobility rules).
The Schwille group aims to (re)construct cellular processes, and ultimately minimal living cells, from dramatically simplified functional subsystems, such as proteins and protein assemblies. Besides being able to investigate biomolecular processes with unprecedented precision in cell-free environments, this approach will also provide insights about the emergence of complexity as a main driver of biological evolution. We have in the past accomplished a thorough biochemical mastering of a class of self-organizing, pattern-forming bacterial proteins that can be harnessed to precisely position proteins and other surface-bound molecules on biological membranes. As membrane-less condensates have recently shown to act as local membrane-active agents, we will explore how and whether different kinds of biological condensates can be actively targeted to membrane surfaces and within protocell vesicles. The project aims at tailoring the protein positioning machinery to position both, surface-attached condensate nuclei, and whole condensate droplets on membranes of variable composition, size, and shape.
The project will include mandatory secondments at UC London, UK (Di Michele Group), and at MPI Potsdam (Reinhard Lipowsky), as well as further optional ones.
Requirements: Applicants should hold or expect to attain, as a minimum MSc degree, or equivalent, in Biophysics, Biochemistry, Physics, Chemistry, Molecular biology, Bioengineering or related area. Practical experience with model membranes and/or biomolecular condensates, Fluorescence microscopy and/or spectroscopy, and programming are desirable.
Position/Salary: MSCA Doctoral Networks provide attractive salary terms and benefits, including a living allowance of around €3,450, a mobility allowance of €600 and, a family allowance (€660 if applicable). Note that these are gross values.
Earliest entry date: preferably within the first half of 2025,
Website: https://www.biochem.mpg.de/schwille
Apply here!