High-resolution structural and mechanical studies of molecular motor proteins at the nanoscale
Prof. Claudia Veigel, Cellular Physiology, Biomedical Centre, LMU Munich
Project:
The large family of myosin motor proteins fulfills a myriad of tasks in mammalian health and disease, including in cancers and neuro-degenerative diseases. These tasks range from muscle contraction to intracellular membrane trafficking and sensory functions in mammalian hearing. Understanding their specific functions in the cell, requires in vitro model systems to be established at the single molecule level. The aim of this PhD thesis is to investigate the interactions of the motor proteins with their cargo membranes. The following techniques will be used:
· Single molecule structural studies using cryo-electron microscopy
· High-resolution single molecule, optical tweezers-based mechanical studies
· Interferometric scattering microscopy
· Fluorescence microscopy techniques including confocal, TIRF, FRAP, super-resolution STORM
· Molecular biology and biochemical techniques
Requirements:
Master’s degree in Physics, Biophysics, Biochemistry, Biotechnology
Position/Salary: TV-L E13 75% - earliest entry date: 202501.01.23 - up to 4 years
Website:http://www.cell.physiol.med.uni-muenchen.de/index.html